NBP2-34655-0.1 mg

antibody from Novus Biologicals

Targeting: KRT8 CARD2, CK8, CYK8, K2C8, K8, KO

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-34655-0.1 mg

Provider

Novus Biologicals

Product name

Mouse Monoclonal Cytokeratin 8/18 Antibody

Antibody type

Monoclonal

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (K8.8 + DC10) - Azide and BSA Free [NBP2-34655] - Formalin-fixed, paraffin-embedded human Overian cancer stained with Cytokeratin 8/18 Monoclonal Antibody (K8.8 + DC10).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (K8.8 + DC10) - Azide and BSA Free [NBP2-34655] - Formalin-fixed paraffin-embedded human colon carcinoma stained with cytokeratin 8/18 Monoclonal Antibody (K8.8 +DC10)

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Cytokeratin 8/18 Antibody (K8.8 + DC10) - Azide and BSA Free [NBP2-34655] - An intracellular stain was performed on HeLa cells with Cytokeratin 8/18 Antibody (K8.8 + DC10) NBP2-34655AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Cytokeratin 8/18 Antibody (K8.8 + DC10) - Azide and BSA Free [NBP2-34655] - An intracellular stain was performed on HeLa cells with Cytokeratin 8/18 Antibody (K8.8 + DC10) NBP2-34655R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550. Image using the DyLight 550 form of this antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): Cytokeratin 8/18 Antibody (K8.8 + DC10) - Azide and BSA Free [NBP2-34655] - An intracellular stain was performed on A549 cells with Cytokeratin 8/18 Antibody (K8.8 + DC10) NBP2-34655R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550. Image using the Azide and BSA Free form of this antibody.